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NEB/Hemo KlenTaq®/M0332S/200 reactions
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发布时间:2025-01-10
Description:
Hemo KlenTaq is a truncated version of Taq DNA Polymerase, lacking the first 280 amino acids (1). Hemo KlenTaq also contains mutations that make it resistant to inhibitors present in whole blood. It can amplify directly from whole blood from humans and mice. Hemo KlenTaq tolerates up to 20% whole blood in a 25 µl reaction (30% in 50 µl reaction).
Amplification of human whole blood with Hemo KlenTaq. Percent blood present in sample and anticoagulant used are indicated in the legend. Ladder M is the 2-Log DNA Ladder (NEB #N3200).Product Source
An E. coli strain that carries a mutant Taq DNA polymerase gene. The protein lacks the N-terminal 5´→ 3´ exonuclease domain and the gene has three internal point mutations.Reagents Supplied
The following reagents are supplied with this product:
Store at (°C) | Concentration | |
Hemo KlenTaq® Reaction Buffer Pack | -20 | 5X |
本文链接: http://klentaq.immuno-online.com/view-1561693348.html
发布于 : 2025-01-10
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